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McCloskey Home Page | Medicinal Chemistry
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Abstract
| Nucleosides Nucleotides 1999 Jun-Jul;18(6-7):1549-53. New techniques for the rapid characterization of oligonucleotides by mass spectrometry. McCloskey JA, Whitehill AB, Rozenski J, Qiu F, Crain PF. |
| Recent advances in combined HPLC/electrospray ionization-mass spectrometry provide effective new capabilities for the rapid characterization of oligonucleotides. Accurate mass measurements with errors < 0.3 Da, and determination of base and sugar modification and of nearest neighbor identities, can be routinely carried out on 10-100 component mixtures of RNA or DNA. These Procedures are widely applicable in structural and analytical studies involving mixtures of oligonucleotides.
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