Prestwich Research Group

Glenn D. Prestwich, Ph.D.
Department of Medicinal Chemistry
University of Utah
419 Wakara Way, Suite 205
Salt Lake City, Utah 84108-1257
phone: 801-585-9051
fax: 801-585-9053
gprestwich@pharm.utah.edu

Publications: Journal Articles

Journal Articles(2001-present)

HA and Biomaterials
Isoprenoids
Phospholipids and Lysolipids
Phosphoinositides

HA and Biomaterials

Y. Luo, K.R.Kirker, and G.D. Prestwich, "Cross-Linked Hyaluronic Acid Hydrogel Films: New Biomaterials for Drug Delivery", J.Controlled Rel., 69, 169-184 (2000).A.J. Day and G.D. Prestwich, "Hyaluronan-binding Proteins: Tying Up the Giant", J.Biol.Chem., 277, 4585-4588 (2002). G.D. Prestwich, "Biomaterials from Chemically-Modified Hyaluronan", Glycoforum
http://glycoforum.gr.jp/science/hyaluronan/HA18/HA18E.html(2001).

Y. Luo and G.D. Prestwich, "Novel Biomaterials for Drug Delivery", Exp. Opin. Ther. Patents, 11, 1395-1410 (2001).

M.R. Ziebell, Z.-G. Zhao, B. Luo, Y. Luo, E.A. Turley, and G.D. Prestwich, "Peptides that Mimic Glycosaminoglycans: HighAffinity Ligands for A Hyaluronan Binding Domain", Chem. & Biol., 8, 1081-1084 (2001).

Y. Luo and G.D. Prestwich, "Hyaluronic Acid-N-Hydroxysuccinimide: A Useful Intermediate for Bioconjugation", Bioconjugate Chem., 12, 1085-1088 (2001).

E.L. Chaikof, H. Matthew, J. Kohn, A.G. Mikos, G.D. Prestwich, and C.M. Yip, "Biomaterials and Scaffolds in Reparative Medicine", Ann. New York Acad. Sci., 961, 96-105 (2002).

G.D. Prestwich and H. Matthew, "Hybrid, Composite, and Complex Biomaterials", Ann.New York Acad. Sci., 961, 106-108 (2002).

Y. Luo, N.J. Bernshaw, Z.-R. Lu, J.Kopecek, and G.D. Prestwich, "HPMA Copolymer- Hyaluronan-Doxorubicin Bioconjugates: Targeted Delivery of an Anti-cancer Agent", Pharm. Res., 19, 396-402 (2002).

M.D. Muniruzzaman, A. Marin, Y. Luo, G.D. Prestwich, W.G. Pitt, G. Husseini, and N.Y.Rapoport, "Intracellular Uptake of Pluronic Copolymer: Effect of the Aggregation State", Colloids and Surfaces B: Biointerfaces, 25, 233-241 (2002).

Y. Luo and G.D. Prestwich, "Cancer-Targeted Polymeric Drugs", Curr. Cancer Drug Targ., 2, 209-226 (2002).

M.D. Muniruzzaman, N.Y.Rapoport, A. Marin, Y. Luo, and G.D. Prestwich, "Intracellular Uptake and Trafficking of Pluronic Micelles in Drug-Sensitive and MDR Cells: Effect on the Intracellular Drug Localization", J. Pharm. Sci., 91, 157-170 (2002).

K.R. Kirker, Y. Luo, J.H. Nielson, J. Shelby, and G.D. Prestwich, "Glycosaminoglycan Hydrogel Films as Biointeractive Dressings for Wound Healing", Biomaterials, 23, 3661-3671 (2002).

K.P. Vercruysse, H. Li, Y.Luo, and G.D. Prestwich, "Thermosensitive Lanthanide Complexes of Hyaluronan", Biomacromolecules, 3, 639-643 (2002).

X. Z. Shu, Y. Liu, Y. Luo, M.C. Roberts, and G.D. Prestwich, "Disulfide Crosslinked Hyaluronan Hydrogels",Biomacromolecules, 3, 1304-1311 (2002).

C. Picart, J. Mutterer, L. Richert, Y. Luo, G.D. Prestwich, P. Schaaf, J.-C. Voegel, and P.Lavalle, "Molecular Basis for the Explanation of the Exponential Growth of Polyelectrolyte Multilayers", Proc. Natl. Acad. Sci., USA, 99, 12531-12535 (2002).

X.Z. Shu, Y. Liu, F. Palumbo, and G.D. Prestwich, "Disulfide-Crosslinked Hyaluronan-Gelatin Hydrogel Films: A Covalent Mimic of the Extracellular Matrix for in vitro Cell Growth", Biomaterials, 24, 3825-3834 (2003).

Y. Liu, X.Z. Shu, S.D. Gray, and G.D. Prestwich, "Disulfide-Crosslinked Hyaluronan-Gelatin Sponge:Growth of Fibrous Tissue in vivo", J. Biomed. Mater. Res., 68A, 142-149 (2004).

W.G. Pitt, R.N. Morris, M.L. Mason, M.W. Hall, Y. Luo, and G.D. Prestwich, "Bioactive Implants: Attachment of Hyaluronic Acid to Metallic Surfaces", J. Biomed. Mater. Res., 68A, 95-106 (2004).

K.R. Kirker, Y. Luo, S.E. Morris, J. Shelby, and G.D. Prestwich, "Glycosaminoglycan Hydrogel Films as Supplemental Wound Dressings for Donor Sites", J. Burn Care Rehabil., 25, 276-286 (2004).

X.Z. Shu, Y. Liu, F. Palumbo, Y, Luo, and G.D. Prestwich, "In situ Crosslinkable Glycosaminoglycan Hydrogels for Tissue Engineering", Biomaterials, 25, 1339-1348 (2004).

X.Z. Shu, K. Ghosh, Y. Liu, F.S. Palumbo, Y. Luo, R.A. Clark, and G.D. Prestwich, "Attachment and Spreading of Fibroblasts on an RGD Peptide-Modified Injectable Hyaluronan Hydrogel", J. Biomed. Mat. Res., 68A, 365-375 (2004).

R.A. Peattie, A.P. Nayate, M.A. Firpo, J. Shelby, R.J. Fisher, and G.D. Prestwich, "Stimulation of InVivo Angiogenesis by Cytokine-loaded Hyaluronic Acid Hydrogel Implants", Biomaterials, 25, 2789-2798 (2004).

S. Cai, J.L. Dufner-Beattie, and G.D. Prestwich, "A Selective Protein Sensor for Heparin Detection", Analyt. Biochem., 326, 33-41 (2004).

Y. Liu, K. Webb, K.R. Kirker, N.J.Bernshaw, P.A. Tresco, S.D. Gray, and G.D. Prestwich, "Composite Articular Cartilage Engineered on a Chondrocyte-Seeded Aliphatic Polyurethane Sponge", Tissue Eng., 10, 1084-1092 (2004).

L. Richert, P. Lavalle, E. Payan, X.Z. Shu, G.D. Prestwich, J.-F. Stoltz, P. Schaaf, J.-C.Voegel, and C. Picart, "Layer by Layer Buildup of Polysaccharide Films: Physical Chemistry and Cellular Adhesion Aspect", Langmuir, 20, 448-458 (2004).

K.R. Kirker and G.D. Prestwich, "Physical Properties of Glycosaminoglycan Hydrogels", J.Polym. Sci. Part B: Polym. Phys., 42, 4344-4356 (2004).

M.E Gilbert, K.R. Kirker, S.D. Gray, P.D. Ward, G.D. Prestwich, and R.R. Orlandi, "Chrondroitin Sulfate Hydrogel and Wound Healing in Rabbit Maxillary Sinus Mucosa", Laryngoscope, 114, 1406-1409 (2004).

H. Li, Y. Liu, X.Z. Shu, S.D. Gray, and G.D. Prestwich, "Synthesis and Biological Evaluation of a Cross-Linked Hyaluronan -Mitomycin C Hydrogel", Biomacromolecules, 5, 895-902 (2004).

Q. Chen, S. Cai, K.G. Shadrach, G.D. Prestwich, and J.G. Hollyfield, "Spacrcan Binding to Hyaluronan and Other Glycosaminoglycans: Molecular and Biochemical Studies", J.Biol. Chem., 279, 23142-23150 (2004).

Y. Liu, H. Li, X.Z. Shu, S.D. Gray, G.D. Prestwich, "Crosslinked Hyaluronan Hydrogels Containing Mitomycin C Reduce Post-operative Abdominal Adhesions", Fertil. & Steril., 83, 1275-1283 (2005).

Y. Liu, X.Z. Shu, and G.D. Prestwich, "Biocompatibility and Stability of Disulfide-Crosslinked Hyaluronan Films", Biomaterials, 26, 4737-4746 (2005).

S. Cai, Y. Liu, X.Z. Shu, and G.D. Prestwich, “Injectable Glycosaminoglycan Hydrogels for Controlled Release of Basic Fibroblast Growth Factor,” Biomaterials, 26, 6054-6067 (2005).

V. Mironov, V. Kasyanov, X.Z. Shu, C. Eisenberg, L. Eisenberg, S. Gonda, T. Trusk, R.R. Markwald, and G.D. Prestwich, “Fabrication of Tubular Tissue Constructs by Centrifugal Casting of Cells Suspended in an In Situ Crosslinkable Hyaluronan-Gelatin Hydrogel,” Biomaterials, 26, 7628-7635 (2005).

K. Ghosh, X.Z. Shu, R. Mou, J. Lombardi, G.D. Prestwich, M.H. Rafailovich, and R.A.F. Clark, ”Rheological Characterization of in Situ Cross-Linkable Hyaluronan Hydrogels,” Biomacromolecules, 6, 2857-2865 (2005).

J.K. Hansen, S.L. Thibeault, J.F. Walsh, X.Z. Shu, and G.D. Prestwich, “In vivo Engineering of the Vocal Fold ECM with Injectable HA Hydrogels: Early Effects on Tissue Repair and Biomechanics in a Rabbit Model,” Ann. Otol. Rhinol. Laryngol., 114, 662-670 (2005).

D. Jiang, J. Liang, J. Fan, S. Yu, S. Chen, Y. Luo, G.D. Prestwich, M.M. Mascarenhas, H.G. Garg, D.A. Quinn, R.J. Homer, D.R. Goldstein, R. Bucala, P.J. Lee, R. Medzhitov, and P.W. Noble, “Regulation of Lung Injury and Repair by Toll-like Receptors and Hyaluronan,” Nat. Med., 11, 1173-1179 (2005).

R.A. Peattie, E. Rieke, E. Hewett, R.J. Fisher, X.Z. Shu, and G.D. Prestwich, “Dual Growth Factor-Induced Angiogenesis In Vivo Using Hyaluronan Hydrogel Implants,” Biomaterials, 27, 1868-1875 (2006)

R. Brunecky, S. Lee, P.W. Rzepecki, M. Overduin, G.D. Prestwich, A.G. Kutateladze, and T.G. Kutateladze, “Investigation of the Binding Geometry of a Peripheral Membrane Protein,” Biochemistry, 44, 16064-16071 (2005).

G.D. Prestwich, X.Z. Shu, Y. Liu, S. Cai, J.F. Walsh, C.W. Hughes, K.R. Kirker, R.R. Orlandi, A.H. Park, S.L. Thibeault, and M.E. Smith, “Injectable Synthetic Extracellular Matrices for Tissue Engineering and Repair,” Adv. Exp. Med. Biol., in press (2005).

M. Proctor, K. Proctor, X.Z. Shu, L.D. McGill, G.D. Prestwich, and R.R. Orlandi, “Composition of Hyaluronan Affects Wound Healing in the Rabbit Maxillary Sinus,” Am. J. Rhinol., 20, 206-211 (2006).

K. Ghosh, X.-D. Ren, X.Z. Shu, G.D. Prestwich, and R.A.F. Clark, “Fibronectin Functional Domains Coupled to Hyaluronan Stimulate Adult Human Dermal Fibroblast Responses Critical for Wound Healing,” Tissue Eng., 12, 601-613 (2006).

C. Sondrup, Y. Liu, X.Z. Shu, G.D. Prestwich, and M.E. Smith, “Bioactive Stents in the Prevention of Airway Stenosis,” Otolaryngol. Head & Neck Surg., 135, 28-35 (2006).

S. Duflo, S.L. Thibeault, W. Li, X.Z. Shu, and G.D. Prestwich, “Vocal Fold Tissue Repair in Vivo Using a Synthetic Extracellular Matrix,” Tissue Eng., 12, 2171-2180 (2006).

V. Mironov, V.A. Kasyanov, M.J. Yost, R. Visconti, W. Twal, T. Trusk, X. Wen, I. Ozolanta, A. Kadishs, G.D. Prestwich, L. Terracio, and R.R. Markwald, “Cardiovascular Tissue Engineering I. Perfusion Bioreactors: A Review,” J. Long-Term Eff. Med. Implants, 16, 111-130 (2006).

Y. Liu, S. Ahmad, X.Z. Shu, R.K. Sanders, S.A. Kopesec, and G.D. Prestwich, “Accelerated Repair of Cortical Bone Defects Using a Synthetic Extracellular Matrix to Deliver Human Demineralized Bone Matrix,” J. Orthoped. Res., 24, 1454-1462 (2006).

A.L. Brown, M.J. Ringuette, G.D. Prestwich, D.J. Bagli, and K.A. Woodhouse, “Effects of Hyaluronan and SPARC on Fibroproliferative Events Assessed in an In Vitro Bladder Acellular Matrix Model,” Biomaterials, 27, 3825-3835 (2006).

X.Z. Shu, S. Ahmad, Y. Liu, and G.D. Prestwich, “Synthesis and Evaluation of Injectable, in situ Crosslinkable Synthetic Extracellular Matrices (sECMs) for Tissue Engineering,” J. Biomed. Mater. Res., 79A, 902-912 (2006).

A.H. Park, C.W. Hughes, A. Jackson, L. Hunter, L. McGill, S.E. Simonsen, S.C. Alder, X.Z. Shu, and G.D. Prestwich, “Crosslinked Hydrogels for Tympanic Membrane Repair,” Otolaryngol. Head Neck Surg., in press (2006).

A.H. Park, A. Jackson, L. Hunter, L. McGill, S.E. Simonsen, S.C. Alder, X.Z. Shu, and G.D. Prestwich, “Crosslinked Hydrogels for Middle Ear Packing,” Otol. Neurotol., in press (2006).

G.D. Prestwich, Y. Liu, B. Yu, X.Z. Shu, and A. Scott, “3-D Culture in Synthetic Extracellular Matrices: New Tissue Models for Drug Toxicology and Cancer Drug Discovery,” invited, Adv. Enz. Reg., in press (2006).

Y. Ji, K. Ghosh, X.Z. Shu, B. Li, J.C. Sokolov, G.D. Prestwich, R.A.F. Clark, and M.H. Rafailovich, “Electrospun Three-Dimensional Hyaluronic Acid Nanofibrous Scaffolds,” Biomaterials, 27, 3782-3792 (2006).

T.D. Mehra, K. Ghosh, X.Z. Shu, G.D. Prestwich, and R.A.F. Clark, “Molecular Stenting with a Crosslinked Hyaluronan Derivative Inhibits Collagen Gel Contraction,” J. Invest. Dermatol., 126, 2202-2209 (2006).

S. Duflo, S.L. Thibeault, W. Li, X.Z. Shu, and G.D. Prestwich, “Effect of a Synthetic Extracellular Matrix on Vocal Fold Lamina Propria Gene Expression in Early Wound Healing,” Tissue Eng., in press (2006).

A.L. Brown, E.M. Srokowski, X.Z. Shu, G.D. Prestwich, and K.A. Woodhouse, “Development of a Model Bladder Extracellular Matrix Combining Disulfide Cross-linked Hyaluronan with Decellularized Bladder Tissue,” Macromol. Biosci., 6, 648-657 (2006).

J. Gajewiak, S. Cai, X.Z. Shu, and G.D. Prestwich, “Aminooxy Pluronics: Synthesis and Preparation of Glycosaminoglycan Adducts,” Biomacromolecules, 7, 1781-1789 (2006).

Y. Liu, X.Z. Shu, and G.D. Prestwich, “Reduced Post-operative Intra-abdominal Adhesions Using CarbylanTM-SX, a Semi-synthetic Glycosaminoglycan Hydrogel,” Fertil. & Steril., in press (2006).

D.B. Pike, S. Cai, K.R. Pomraning, M.A. Firpo, R.J. Fisher, X.Z. Shu, G.D. Prestwich, and R.A. Peattie, “Heparin-regulated Release of Growth Factors In Vitro and Angiogenic Response In Vivo to Implanted Hyaluronan Hydrogels Containing VEGF and bFGF,” Biomaterials, 27, 5242-5251 (2006).

Y. Liu, X.Z. Shu, and G.D. Prestwich, “Osteochondral Defect Repair with Autologous Bone Marrow-Derived Mesenchymal Stem Cells in an Injectable, in situ Crosslinked Synthetic Extracellular Matrix,” Tissue Eng., 12, (2006).

G.D. Prestwich and J.-w. Kuo, "Chemically-Modified HA for Therapy and Regenerative Medicine,” invited article, Curr. Pharm. Biotech., in press (2006).

C.M. Riley, P.W. Fuegy, M.A. Firpo, X.Z. Shu, G.D. Prestwich, and R.A. Peattie, (back to top)

Isoprenoids

H.-K. Lee, P. Denner-Ancona, J. Sakakibara, T. Ono, and G.D. Prestwich, "Photoaffinity Labeling and Site-Directed Mutagenesis of Rat Squalene Epoxidase", Arch.Biochem. Biophys., 381, 43-52 (2000).T. Dang and G.D. Prestwich, "Site-directed Mutagenesis of Squalene-Hopene Cyclase: Altered Substrate Specificity and Product Distribution," Chem. & Biol., 7, 643-649 (2000).X.-h. Liu and G.D. Prestwich, "Didehydrogeranylgeranyl (ￇG): A Fluorescent Probe for Protein Prenylation",J. Am. Chem. Soc., 124, 20-21 (2002).

X. Li, M.P. Rivas, M. Fang, J. Marchena, B. Mehrotra, A. Chaudhary, L. Feng, G.D.Prestwich, and V.A. Bankaitis, "Analysis of Oxysterol Binding Protein Homologue Kes1p Function in Regulation of Sec14p-dependent Protein Transport from the Yeast Golgi Complex", J. Cell Biol., 157, 63-77 (2002).

J.C. Hinshaw, D.-Y. Suh, P. Garnier, F.S. Buckner, R.T. Eastman, S.P.T. Matsuda, B.M.Joubert, I. Coppens, K.A. Joiner, S. Merali, T.E. Nash, and G.D. Prestwich, "Oxidosqualene Cyclase Inhibitors as Antimicrobial Agents", J. Med. Chem., 46, 4240-4243 (2003).

H. Zhang, X.-h. Liu, K. Zhang, C.-K. Chen, J.M. Frederick, G.D. Prestwich, and W.Baehr, "Photoreceptor cGMP Phosphodiesterase ￓubunit (PDE￠Functions as a Prenyl-binding Protein", J. Biol. Chem., 279, 407-413 (2004).

P.Y. Lum, C.D. Armour, S.B. Stepaniants, G. Cavet, M.K. Wolf, J.S. Butler, J.C. Hinshaw, P. Garnier, G.D. Prestwich, A. Leonardson, P.Garrett-Engele, C.M. Rush, M. Bard, G.Shimmack, J.W. Philips, C.J.Roberts, and D.D. Shoemaker, "Discovering Modes of Action for Therapeutic Compounds Using a Genome-Wide Screen of Yeast Heterozygotes", Cell, 116, 121-137 (2004).

X.-h. Liu, D.-Y. Suh, J. Call, and G.D. Prestwich, "Antigenic Prenylated Peptide Conjugates and Polyclonal Antibodies to Detect Protein Prenylation", Bioconjugate Chem., 15, 270-277 (2004).

H.-K. Lee, Y.F. Zheng, X.-y. Xiao, M. Bai, J. Sakakibara, T.Ono, and G.D. Prestwich, "Photoaffinity Labeling Identifies the Substrate-Binding Site of Mammalian Squalene Epoxidase," Biochem. Biophys. Res. Commun., 315, 1-9 (2004).X.-h. Liu and G.D. Prestwich, "Didehydrofarnesyl Diphosphate: An Intrinsically Fluorescent Inhibitor of Protein Farnesyltransferase", Bioorg. Med. Chem. Lett., 14, 2137-2140 (2004).

M.R. Ziebell and G.D. Prestwich, "Specificity Determinants of Carbohydrate Mimics Evaluated by NMR and Molecular Modeling", J. Computer-aided Molec. Design, 18,597-614 (2005).

M. Proctor, K. Proctor, X.Z. Shu, L.D. McGill, G.D. Prestwich, and R.R. Orlandi, "Composition of Hyaluronan Affects Wound Healing in the Rabbit Maxillary Sinus", Am. J. Rhinology, inpress (2005).

H. Zhang, K. Shibuya, H. Hemmi, T. Nishino, and G.D. Prestwich, “Total Synthesis of Geranylgeranylglyceryl Phosphate Enantiomers: Substrates for Characterization of 2,3-O-Digeranylgeranylglyceryl Phosphate Synthase,” Organic Lett., 8, 943-946 (2006).

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Phospholipids and Lysolipids

S.S. Davies, A.V. Pontsler, G.K. Marathe, K.A. Harrison, R.C. Murphy, J.C. Hinshaw, G.D.Prestwich, A. St. Hilaire, S.M. Prescott, G.A. Zimmerman, and T.M. McIntyre, "Oxidized Alkyl Phospholipids are Specific, High Affinity Peroxisome Proliferator-activated Receptor ￊLigands and Agonists," J. Biol. Chem., 276, 16015-16023 (2001).

L. Feng, K. Manabe, J.C. Shope, S. Widmer, D.B. DeWald, and G.D. Prestwich, "AReal-Time Fluorogenic Phospholipase A2 Assay for Biochemical and Cellular Activity Measurements", Chem. & Biol., 9, 795-803 (2002).

Y. Xu and G.D. Prestwich, "Concise Synthesis of Acyl Migration-Blocked 1,1-Difluorinated Analogues of Lysophosphatidic Acid", J. Org. Chem., 67, 7158-7161 (2002).

Y. Xu and G.D. Prestwich "Synthesis of Chiral (,-Difluoroalkyl)phosphonate Analogues of (Lyso)phosphatidic Acid via Hydrolytic Kinetic Resolution", Organic Lett., 4, 4021-4024 (2002).

T.M.McIntyre, A.V. Pontstler, A.R. Silva, A. St. Hilaire, Y. Xu, J.C. Hinshaw, G.A.Zimmerman, K. Hama, J. Aoki, H. Arai, and G.D.Prestwich, "Identification of an Intracellular Receptor for Lysophosphatidic Acid (LPA): LPA is a Transcellular PPAR￁gonist", Proc. Natl. Acad. Sci., USA, 100, 131-136 (2002).

Y. Xu, L. Qian, and G. D. Prestwich, "Synthesis of Monofluorinated Analogues of Lysophosphatidic Acid", J. Org. Chem., 68, 5320-5330 (2003).

Y. Xu, L. Qian, and G.D. Prestwich, "Synthesis of -Fluorinated Phosphonates from-Fluorovinylphosphonates: A New Route to Analogues of Lysophosphatidic Acid", Organic Lett., 5, 2267-2270 (2003).

S.S. Smyth, V.A. Sciorra, Y.A. Sigal, Z. Wang, Y. Xu, G.D. Prestwich, and A.J. Morris, "Lipid Phosphate Phosphatase 1 (LPP1) Regulates Lysophosphatidic Acid Signaling in Platelets", J. Biol. Chem., 278, 43214-43223 (2003).

L. Feng, G.B. Mills, and G.D. Prestwich, "Modulators of Lysophosphatidic Acid Signaling", Expert Opin. Therapeut. Pat., 13, 1619-1634 (2003)

.L. Qian, Y. Xu, H. Arai, J. Aoki, T.M. McIntyre, and G.D. Prestwich, "Synthesis of Migration-Resistant Hydroxyethoxy Analogs of Lysophosphatidic Acid", Organic Lett., 5, 4685-4688 (2003).

L. Qian, Y. Xu, Y. Hasegawa, J. Aoki, G.B. Mills, and G.D. Prestwich, "Enantioselective Responses to a Phosphorothioate Analogue of Lysophosphatidic Acid with LPA3 Receptor-Selective Agonist Activity", J. Med. Chem., 46, 5575-5578 (2003).

Y. Xu, L. Qian, A.V. Pontsler, T.M. McIntyre, and G.D. Prestwich, "Synthesis of Difluoromethyl Substituted Lysophosphatidic Acid Analogues",Tetrahedron, 60, 43-49 (2004).

H. Zhou, H. Xiong, H.X Li, S.E. Plevy, P.D. Walden, M. Sassaroli, G.D. Prestwich, and J.C.Unkeless, "Microtubule-assocated serine/threonine Kinase-205 kDa and Fcￒeceptor Control IL-12 p40 Synthesis and NF-ￂ Activation", J. Immunol., 172, 2559-2568 (2004).

M. Umezo-Goto, J. Tanyi, J. Lahad, S. Liu, S. Yu, R. Lapushin, Y. Hasegawa, Y. Lu, R.Trost, T.Bevers, E. Jonasch, K. Aldape, J. Liu, R.D. James, C.G. Ferguson, Y. Xu, G.D.Prestwich, and G.B. Mills, “Lysophosphatidic Acid Production and Action: Validated Targets in Cancer?,” J.Cell. Biochem., 92, 1115-1140 (2004).

Y. Xu, Y. Fang, J. Chen, and G.D. Prestwich, "Activation of mTOR Signaling by Novel Fluoromethylene Phosphonate Analogues of Phosphatidic Acid", Bioorg. Med. Chem. Lett., 14, 1461-1464 (2004).

C. Zhang, D.L. Baker, S. Yasuda, N. Makarova, L.R. Johnson, L. Balazs, G.K. Marathe, T.M.McIntyre, Y. Xu, G.D. Prestwich, H.-S. Byun, R. Bittman, and G. Tigyi, "LysophosphatidicAcid Induces Neointima Formation Through PPAR￁ctivation", J.Exper. Med., 199, 763-774 (2004).

R. Schneiter, B. Br￧er, C.M. Amann, G.D. Prestwich, R.F. Epand, G. Zellnig, F.T.Wieland, and R.M. Epand, "Identification and Biophysical Characterization of a Very Long-chain Fatty Acid-substituted Phosphatidylinositol in Yeast Subcellular Membranes", Biochem. J., 381, 941-949 (2004).

Y. Xu, M. Tanaka, H. Arai, J. Aoki, and G.D. Prestwich, "Alkyl Lysophosphatidic Acid and Fluoromethylene Phosphonate Analogs as Metaboically-Stabilized Agonists for LPA Receptors", Bioorg. Med. Chem. Lett., 14, 5323-5328 (2004).

Y. Xu, J. Aoki, K. Shimizu, M. Umezu-Goto, K. Hama, T. Takanezawa, S. Yu, G.B. Mills, H. Arai, L. Qian, and G.D. Prestwich, “Structure-activity Relationships of Fluorinated Lysophosphatidic Acid Analogues,” J. Med. Chem., 48, 3319-3327 (2005).

G.D. Prestwich,Y. Xu, L. Qian, J. Gajewiak, and G. Jiang, “New Metabolically Stabilized Analogues of Lysophosphatidic Acid: Agonists, Antagonists, and Enzyme Inhibitors,” Biochem. Soc. Trans., 33, 1357-1361 (2005).

S. Desmaret, L. Qian, B. Vanloo, K. Meerschaert, J. Van Damme, J. Grooten, J. Vandekerckhove, G.D. Prestwich, and J. Gettemans, “Lysophosphatidic Acid Lipidomics Reveals an Interaction Between Pyruvate Kinase and Clathrin Heavy Chain/Hsp70,” Biol. Chem., in press (2005).

G. Jiang, Y. Xu, T. Falgui￥s, J. Gruenberg, and G.D. Prestwich, “Concise Synthesis of Ether Analogues of Lysobisphosphatidic Acid,” Organic Lett., 7, 3837-3840 (2005).

G.D. Prestwich, “Visualization and Perturbation of Phosphoinositide and Phospholipid Signaling,” Prostaglandins Other Lipid Mediat., 77, 168-178 (2005).

Y. Xu, X.-h. Liu, and G.D. Prestwich, “Synthesis of Phosphatase-resistant Analogues of Phytic Acid (InsP6),” Tetrahedron Lett., 46, 8311-8314 (2005).

G. Jiang, Y. Xu, and G.D. Prestwich, “Practical Enantiospecific Syntheses of Lysobisphosphatidic Acid and Phosphorothioate Analogues,” J. Org. Chem., in press (2005).

S. Desmaret, L. Qian, B. Vanloo, K. Meerschaert, J. Van Damme, J. Grooten, J. Vandekerckhove, G.D. Prestwich, and J. Gettemans, “Lysophosphatidic Acid Affinity Chromatography Reveals Pyruvate Kinase as a Specific LPA-Binding Protein,” Biol. Chem., 386, 1137-1147 (2005).

L. Qian, Y. Xu, T.D. Simper, G. Jiang, J. Aoki, M. Umezo-Goto, H. Arai, S. Yu, G.B. Mills, R. Tsukahara, N. Makarova, Y. Fujiwara, G. Tigyi, and G.D. Prestwich, “Phosphorothioate Analogues of Alkyl Lysophosphatidic Acid LPA3 Receptor-Selective Agonists,” Chem. Med. Chem., 1, 376-383 (2006).

J. Ren, Y.-j. Xiao, L.S. Singh, L. Feng, T.M. Rose, G.D. Prestwich, and Y. Xu, “Human Peritoneal Mesothelial Cells Constitutively Produce LPA in Their Conditional Media, which Enhance Cell Adhesion, Migration, and Invasion of Ovarian Cancer Cells,” Cancer Res., 66, 3006-3014 (2006).

T.M. Rose and G.D. Prestwich, “Fluorogenic Phospholipids as Head Group-Selective Reporters of Phospholipase A Activity,” ACS Chemical Biology, 1, 63-92 (2006).

G. Jiang, Y. Xu, and G.D. Prestwich, “Practical Enantiospecific Syntheses of Lysobisphosphatidic Acid and Phosphorothioate Analogues,” J. Org. Chem., 71, 934-939 (2006).

C.G. Ferguson, C.S. Bigman, R.D. Richardson, L.A. van Meeteren, W.H. Moolenaar, and G.D. Prestwich, “Fluorogenic Phospholipid Substrate to Detect Lysophospholipase D/Autotaxin Activity,” Organic Lett., 8, 2023-2026 (2006).

T.M. Rose and G.D. Prestwich, “Synthesis and Evaluation of Fluorogenic Substrates for Phospholipase D and Phospholipase C,” Organic Lett., 8, 2575-2578 (2006).

H. Zhang, R. Tsukuhara, G. Tigyi, and G.D. Prestwich, “Synthesis of Cyclic Phosphate Analogues of (Lyso)phosphatidic Acid Using a Ring-Closing Metathesis Reaction,” J. Org. Chem., 71, 6061-6066 (2006).

Y. Xu, G. Jiang, R. Tsukahara, Y. Fujiwara, G. Tigyi, and G.D. Prestwich, “Phosphonothioate and Fluoromethylene Phosphonate Analogues of Cyclic Phosphatidic Acid: Novel Antagonists of Lysophosphatidic Acid Receptors,” J. Med. Chem., 49, 5309-5315 (2006).

X. Zhao, D. Wang, Z. Zhao, S. Sengupta, Y. Xiao, R. Zhang, K. Lauber, S. Wesselborg, L. Feng, T.M. Rose, Y. Shen, Y. Shen, J. Zhang, G.D. Prestwich, and Y. Xu, “Caspase-3-dependent Activation of Calcium-independent Phospholipase A2 Enhances Cell Migration in Non-apoptotic Ovarian Cancer Cells,” J. Biol. Chem., 281, 29357-29368 (2006).

J. Gajewiak and G.D. Prestwich, “Phosphomimetic Sulfonamide and Sulfonamidoxy Analogues of (Lyso)Phosphatidic Acid,” Tetrahedron Lett., 47, 7607-7609 (2006).

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Phosphoinositides

D.B. DeWald, H. Hama, C. Jones, J. Thompson, A. Cangelosi, and G.D. Prestwich, "Rapid Accumulation of Phosphatidylinositol 4,5-Bisphosphate and Inositol 1,4,5-Trisphosphate Correlates with Calcium Mobilization in Salt-Stressed Arabidopsis", PlantPhysiol., 126, 759-769 (2001).

S. Ozaki, D.B. DeWald, J.C. Shope, J. Chen, and G.D. Prestwich, "Intracellular Delivery of Phosphoinositides and Inositol Phosphates using Polyamine Carriers", Proc. Natl. Acad. Sci., USA, 97, 11286-11291 (2000).

L. Feng, M. Mejillano, H.L. Yin, J. Chen, and G.D. Prestwich, "Full-Contact Domain Labeling: Identification of a Novel Phosphoinositide Binding Site on Gelsolin That Requires the Complete Protein", Biochemistry, 40, 904-913 (2001).

M.P. Scheid, M. Huber, J.E. Damen, M. Hughes, V.H. Kang, P.O. Neilsen, G.D.Prestwich, G.Krystal, and V. Duronio, "Phosphatidylinositol(3,4,5)P3 is Essential But Not Sufficient forPKB Activation: Phosphatidylinositol(3,4)P2 is Required for PKB Phosphorylation at Ser473. Studies Using Cells from SHIP Knockout Mice", J. Biol. Chem., 277, 9027-9035 (2002).

G.D. Prestwich, R. Chen, L. Feng, S. Ozaki, C.G. Ferguson, B.E. Drees, D.W.Neklason, M.J.Mostert, P.A. Porter-Gill, V.H. Kang, J.C.Shope, P.O. Neilsen, and D.B. DeWald, "In Situ Detection of Phospholipid and Phosphoinositide Metabolism",Advan. Enzyme Regul., 42, 19-38 (2002).

G.S. Kular, A. Chaudhary, G.D. Prestwich, P. Swigart, S. Cockcroft, and R. Wetzker, "Co-operationofPhosphatidylinositol Transfer Protein with Phosphoinositide 3-Kinase ￩n theGeneration of 3-Phosphorylated Inositol Lipids", Advan. Enzyme Regul. 42, 53-61 (2002).

R. Chen, V.H. Kang, J. Chen, J.C. Shope, J. Torabinejad, D.B. DeWald, and G.D.Prestwich, "AMonoclonal Antibody to Visualize PtdIns(3,4,5)P3 in Cells", J.Histochem. Cytochem., 50, 697-708 (2002).

P. Whitley, A.M. Gibbard, F. Koumanov, S. Oldfield, E.E. Kilgour, G.D. Prestwich, and G.D. Holman, "Identification of Centaurin-ﾺ A Phosphatidylinositide-Binding Protein Present in Fat, Heart, and Skeletal Muscle", Eur. J. Cell. Biol., 81, 222-230 (2002).

M.E. Rauch, C.G. Ferguson, G.D. Prestwich, and D.S. Cafiso, "Myristoylated Alanine-rich C Kinase Substrate (MARCKS) Sequesters Spin-labeled Phosphatidylinositol 4,5-Bisphosphate in Lipid Bilayers", J. Biol. Chem., 277, 14068-14076 (2002).

P.W. Rzepecki and G.D. Prestwich, "Synthesis of Hybrid Lipid Probes: Derivatives ofPhosphatidylethanolamine-Extended Phosphatidylinositol 4,5-Bisphosphate (Pea-PIP2)", J. Org. Chem., 67, 5454-5460 (2002).

O.D. Weiner, P.O. Neilsen, G.D. Prestwich, M.W. Kirschner, L.C. Cantley, and H.R.Bourne, "A PI(3,4,5)P3/Rho GTPase Positive Feedback Loop Regulates Neutrophil Polarity", Nature Cell Biol., 4, 509-513 (2002).

M. Inoue, Y. Sakamoto, N. Fujishiro, I. Imanaga, S. Ozaki, and G.D. Prestwich, "Central and Peripheral Ca2+ Stores in Adrenal Chromaffin Cells", Cell Calcium, 33, 19-26 (2003).

O. Gozani, P. Karuman, D.R. Jones, D. Ivanov, J. Cha, A.A. Lugovskoy, C.L. Baird, H.Zhu, S.J. Field, S.L.Lessnick, J.Villasenor, B.Mehrotra, J.Chen, V.R. Rao, J.S.Brugge, C.G. Ferguson, B. Payrastre, D.G.Myszka, L.C.Cantley, G. Wagner, NDivecha, G.D.Prestwich, and J.Yuan, "The PHD Finger of the Chromatin-Associated Protein ING2 Functions as a Nuclear Phosphoinositide Receptor",Cell, 114, 99-111 (2003).

J.R. Couchman, S. Vogt, S.-T. Lim, E.-S. Oh, G.D. Prestwich, A.B. Theibert, W. Lee, and A.Woods, "Regulation of Inositol Phospholipid Binding and Signaling Through Syndecan-4", J.Biol.Chem., 277, 49296-49303 (2002).

D. McV. Ward, S.L. Shiflett, D.Huynh, M.B Vaughn, G.D. Prestwich, and J.Kaplan, "Use of Expression Constructs to Dissect the Functional Domains of the CHS/Beige Protein: Identification of Multiple Phenotypes", Traffic, 4, 403-415 (2003).

B.E. Drees, A. Weipert, H. Hudson, C.G. Ferguson, L. Chakravarty, and G.D. Prestwich, "Competitive Fluorescence Polarization Assays for the Detection of Phosphoinositide Kinaseand Phosphatase Activity", Comb. Chem. High Throughput Screen, 6, 321-330 (2003).

F. Lemtiri-Chlieh, E.A.C. MacRobbie, A.A.R. Webb, N.F. Manison, C. Brownlee, J.N.Skepper, J.Chen, G.D. Prestwich, and C.A.Brearley, "Inositol Hexakisphosphate Mobilizes an Endomembrane Store of Calcium in Guard Cells," Proc. Natl. Acad. Sci., USA, 100, 10091-10095 (2003).T.G. Kutateladze, D.D.G.S. Capelluto, C.G. Ferguson, M.L. Cheever, G.D. Prestwich, and M. Overduin, "Multivalent Mechanism of Membrane Insertion by the FYVE Domain", J.Biol. Chem., 279, 3050-3057 (2004).

G.D. Prestwich, "Visualizing Signalling by Phosphoinositide 3-Kinase Pathway Lipids", Biochem. Soc. Trans., 32, 336-337 (2004).

B.E. Drees, G.B. Mills, C. Rommel, and G.D. Prestwich, "Therapeutic Potential of Phosphoinositide3-Kinase Inhibitors", Expert Opin. Therapeut. Pat., 14, 703-732(2004).

G.D. Prestwich, “Phosphoinositide Signaling: From Affinity Probes to Pharmaceutical Targets,” Chem. & Biol., 11, 619-637 (2004).

O. Gozani, S.J. Field, C.G. Ferguson, C. Mahlke, L.C. Cantley, G.D. Prestwich, and J.Yuan, "Visualization of Phosphoinositide Action in the Nucleus", Adv. Enz. Reg., inpress (2004).

G. Sweeney, R.R. Garg, R.B. Ceddia, D. Li, M. Ishiki, R. Somwar, L. Foster, P.O. Neilsen, G.D. Prestwich, A. Rudich, and A. Klip,"Intracellular Delivery of Phosphatidylinositol (3,4,5)-Trisphosphate Causes Incorporation of GLUT4 into the Plasma Membrane of Muscle and Fat Cells without Increasing Glucose Uptake", J. Biol. Chem., 279, 32233-32242 (2004).

H. Hama, J. Torabinejad, G.D. Prestwich, and D.B. DeWald, "Measurement and Immunofluorescence of Cellular Phosphoinositides", Methods Mol. Biol., 284, 243-258(2004).

H. Lin, S. Ozaki, N. Fujishiro, K. Takeda, I. Imanaga, G.D. Prestwich, and M. Inoue, "Subunit Composition and Role of Na+, K+-ATPases in Adrenal Chromaffin Cells", J. Physiol., 564, 161-172 (2005).

S.K Elkin, D. Ivanov, M. Ewalt, C.G. Ferguson, S.G. Hyberts, Z.-Y. J. Sun, G.D. Prestwich, J. Yuan, G. Wagner, M.A. Oettinger, and O. Gozani, “A PHD Finger Motif in the C Terminus of RAG2 Modulates Recombination Activity,” J. Biol. Chem., 280, 28701-28710 (2005).

D.B. DeWald, S. Ozaki, S. Malaviya, J.C. Shope, K. Manabe, L. Crosby, P. Neilsen, D. Johnston, S. Harihar, and G.D. Prestwich, “Cellular Calcium Mobilization in Response to Phosphoinositide Delivery,” Cell Calcium, 38, 59-72 (2005).

C.G. Ferguson, R.D. James, C.S. Bigman, D.A. Shepard, Y. Abdiche, P.S. Katsamba, D.G. Myszka, and G.D. Prestwich, “Phosphoinositide-Containing Polymerized Liposomes: Stable Membrane-Mimetic Vesicles for Protein-Lipid Binding Analysis,” Bioconjugate Chem., 16, 1475-1483 (2005).

R. Brunecky, S. Lee, P.W. Rzepecki, M. Overduin, G.D. Prestwich, A.G. Kutateladze, and T.G. Kutateladze, “Investigation of the Binding Geometry of a Peripheral Membrane Protein,” Biochemistry, 44, 16064-16071 (2005).

Y. Xu, S.A. Lee, T.G. Kutateladze, D. Sbrissa, A. Shisheva, and G.D. Prestwich, “Chemical Synthesis and Molecular Recognition of Phosphatase-Resistant Analogues of Phosphatidylinositol-3-phosphate,” J. Am. Chem. Soc., 128, 885-897 (2006).

H. Zhang, Y. Xu, Z. Zhang, E.R. Liman, and G.D. Prestwich, “Synthesis and Biological Activity of Phospholipase C-Resistant Analogues of Phosphatidylinositol 4,5-bisphosphate,” J. Am. Chem. Soc., 128, 5642-5643 (2006).

J. Gajewiak, Y. Xu, S.A. Lee, T.G. Kutateladze, and G.D. Prestwich, “Synthesis and Molecular Recognition of Phosphatidylinositol-3-methylenephosphate,” Organic Lett., 8, 2811-2813 (2006).

H. Zhang, N. Markadieu, R. Beauwens, C. Erneux, and G.D. Prestwich, “Synthesis and Biological Activity of PTEN-Resistant Analogues of Phosphatidylinositol 3,4,5-trisphosphate,” J. Am. Chem. Soc., in press (2006).

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